Lipid Peroxidation (4-HNE) Assay Kit ab238538 from Abcam

Product Specs
ItemLipid Peroxidation (4-HNE) Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab238538
Quantity32 TEST, 96 TEST
Sample TypeSerum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
ApplicationsCellular Assay
ReactivityVertebrata
TypeCompetitive
Detection Target4 Hydroxynonenal

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(1) RNA helicase DDX5 modulates sorafenib sensitivity in hepatocellular carcinoma via the Wnt/β-catenin–ferroptosis axisCell Death & DiseaseNovember 30, 2023Zhili Li, Claude Caron de Fromentel, Woojun Kim, Wen-Hung Wang, Jiazeng Sun, Bingyu Yan, Sagar Utturkar, Nadia Atallah Lanman, Bennett D. Elzey, Yoon Yeo, et al.590 nm. Lipid peroxidation assays Malondialdehyde (MDA) (ab233471) and 4-hydroxynonenal (4-HNE) (ab238538) assays were carried out as described by manufacturer (Abcam). Cell viability assays HCC cells (1

(2) Efficacy of a Spearmint (Mentha spicata L.) Extract as Nutritional Support in a Rat Model of Hypertensive GlaucomaTranslational Vision Science & TechnologyNovember 2, 2023Rosario Amato, Alessio Canovai, Alberto Melecchi, Samanta Maci, Filipa Quintela, Brenda A. Fonseca, Maurizio Cammalleri, Massimo Dal Monteavailable kits: Lipid Peroxidation (MDA) Assay Kit (ab118970, Abcam), Lipid Peroxidation (4-HNE) Assay Kit (ab238538, Abcam), 8-Hydroxy 2 Deoxyguanosine ELISA Kit (ab201734, Abcam), and GSH+GSSG/GSH Assay Kit (Colorimetric)

(3) HMGB1 induces hepcidin upregulation in astrocytes and causes an acute iron surge and subsequent ferroptosis in the postischemic brainExperimental & Molecular MedicineNovember 1, 2023Dashdulam Davaanyam, Hahnbi Lee, Song-I Seol, Sang-A Oh, Seung-Woo Kim, Ja-Kyeong Lee(MDA) Assay Kit (Sigma‒Aldrich, Cat #: MAK085) and Lipid Peroxidation (4-HNE) Assay Kit (Abcam, Cat #: ab238538) according to the manufacturer’s instructions. Briefly, the MDA in the brain tissue was reacted with

(4) The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in gliomaCell Death & DiseaseOctober 23, 2023Zhangjie Wang, Yuan Xia, Yang Wang, Ruiqiu Zhu, Hongbo Li, Yu Liu, Na Shenthe Lipid Peroxidation MDA Assay Kit (ab118970, Abcam) and the Lipid Peroxidation 4-HNE Assay Kit (ab238538, Abcam). The Reactive Oxygen Species (ROS) Assay Kit (S0033M, Beyotime) was used to measure the level

(5) TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signallingJHEP reports : innovation in hepatologyMay 1, 2023Su W, Gao W, Zhang R, Wang Q, Li L, Bu Q, Xu Z, Liu Z, Wang M, Zhu Y, et al.measured using an MDA Assay kit (Cat#S0131S, Beyotime, China), Lipid Peroxidation (4-HNE) Assay Kit (cat#ab238538, Abcam), SOD Assay Kit (Cat#S0109, Beyotime), and GSH/GSSG Assay Kit (Cat#S0053, Beyotime), respectively,

(6) Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathwayCell death & diseaseApril 6, 2023Hao J, Chen Q, Feng Y, Jiang Q, Sun H, Deng B, Huang X, Guan J, Chen Q, Liu X, et al.MDA-TBA adduct can be quantified colorimetrically (OD = 532 nm). A Lipid Peroxidation (4-HNE) Assay Kit (ab238538; Abcam) was used to evaluate the concentration of 4-HNE according to the manufacturer’s protocol. Cell

(7) Does Oxidative Stress Along with Dysbiosis Participate in the Pathogenesis of Asthma in the Obese?Cell Biochemistry and BiophysicsMarch 1, 2023Paulina Kleniewska, Rafał Pawliczakreagents were purchased from Sigma-Aldrich-Poland. A lipid peroxidation (4-HNE) Assay Kit (Item No. ab238538) and a Hydrogen Peroxide Assay Kit (Item No. ab102500) were obtained from ABCAM. An 8-Isoprostane ELISA Kit

(8) Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexiabioRxiv : the preprint server for biologyFebruary 18, 2023Ferrer M, Mourikis N, Davidson EE, Kleeman SO, Zaccaria M, Habel J, Rubino R, Flint TR, Connell CM, Lukey M, et al.of 4-HNE-protein adducts in lysates was performed using the Lipid Peroxidation (4-HNE) Assay Kit (#ab238538; Abcam). Detection of NADPH levels in the adrenal glands was performed using the NADP/NADPH-Glo™ Bioluminescent Assay

(9) Nicotinamide Mononucleotide Administration Prevents Doxorubicin-Induced Cardiotoxicity and Loss in Physical Activity in MiceCellsDecember 27, 2022Marielle Margier, Chisaka Kuehnemann, Nicolas Hulo, Jazmin Morales, Prasanna Vadhana Ashok Kumaar, Cecile Cros, Helene Cannelle, Julie Charmetant, Eric Verdin, Matthias Canault, et al.protein adducts in gastrocnemius muscle lysates were measured using a 4-HNE ELISA kit (Abcam, Product# ab238538) according to the manufacturer’s protocols. 4-HNE quantification was normalized to proteins content

(10) Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related macular degenerationNature CommunicationsDecember 1, 2022Jen-Zen Chuang et al.described81. 4-HNE concentration was quantified using an ELISA kit following the manufacturer’s protocol (#Ab238538, Abcam). The ELISA data analysis was collected by SPECTRA Max (450 nm) M2e plate reader (Molecular Devices

(11) COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolismCell death & diseaseNovember 23, 2022Feng Y, Xu J, Shi M, Liu R, Zhao L, Chen X, Li M, Zhao Y, Chen J, Du W, et al.Peroxidation (MDA) Assay Kit (MAK085, Sigma-Aldrich, USA), and Lipid Peroxidation (4-HNE) Assay Kit (ab238538, Abcam, USA) according to the manufacturer’s instructions. BODIPY staining For BODIPY staining, H838

(12) ATF3 Regulates Osteogenic Function by Mediating Osteoblast Ferroptosis in Type 2 Diabetic OsteoporosisDisease MarkersOctober 26, 2022Yantao Zhao, Yunxia Du, Yijie Gao, Zhijie Xu, Dexiang Zhao, Maowei Yangwere detected by malondialdehyde (MDA) assay kits (Beyotime Biotechnology, 532 nm), 4-HNE assay kits (ab238538, Abcam Inc., Cambridge, MA, USA, 450 nm), and a fluorescent lipid peroxidation probe (C11 BODIPY 581/591,

(13) Dimethylarginine dimethylaminohydrolase 1 protects PM2.5 exposure-induced lung injury in mice by repressing inflammation and oxidative stressParticle and Fibre ToxicologyOctober 14, 2022Junling Gao, Tong Lei, Hongyun Wang, Kai Luo, Yuanli Wang, Bingqing Cui, Zhuoran Yu, Xiaoqi Hu, Fang Zhang, Yingjie Chen, et al.mouse TNFα, IL-6, 3′-NT and 4-HNE were purchased from Abcam PLC (#ab208348, #ab100712, #ab116691 and #ab238538, Cambridge, UK). The Masson’s trichrome staining kit was obtained from Solarbio Science & Technology

(14) Inhibition of cannabinoid receptor type 1 sensitizes triple-negative breast cancer cells to ferroptosis via regulating fatty acid metabolismCell death & diseaseSeptember 21, 2022Li P, Lin Q, Sun S, Yang N, Xia Y, Cao S, Zhang W, Li Q, Guo H, Zhu M, et al.adduct can be quantified colorimetrically (OD = 532 nm). Lipid Peroxidation (4-HNE) Assay Kit (Abcam, ab238538) were used to evaluate the concentration of 4-HNE according to the manufacturer’s protocol. Generation

(15) Genetic and Pharmacological Inhibition of GCN2 Ameliorates Hyperglycemia and Insulin Resistance in Type 2 Diabetic MiceAntioxidants (Basel)August 16, 2022Juntao Yuan, Fang Li, Xiyue Shen, Junling Gao, Zhuoran Yu, Kai Luo, Bingqing Cui, Zhongbing Lu3-nitrotyrosine (3′-NT) and 4-hydroxynonenal (4-HNE) ELISA kits were purchased from Abcam (#ab116691 and #ab238538, Cambridge, UK). Protease and phosphatase inhibitor cocktails were obtained from Roche (#04693124001,

(16) Translating PUFA omega 6:3 ratios from wild to captive hibernators (Urocitellus parryii) enhances sex-dependent mass-gain without increasing physiological stress indicatorsJournal of Comparative Physiology BJuly 1, 2022Monica Mikes, Sarah A. Rice, Doug Bibus, Alexander Kitaysky, Kelly L. Drewwere frozen at − 80 °C. Lysates were diluted 2 × and run with a 4-hydroxynonenal (4-HNE) assay kit (ab238538, Abcam, Cambridge, MA) on an Epoch microplate reader (BioTek Inc., Winooski, VT) at an absorbance of

(17) Dihydroquercetin Attenuates Silica-Induced Pulmonary Fibrosis by Inhibiting Ferroptosis Signaling PathwayFrontiers in PharmacologyMay 12, 2022Leyong Yuan, Yan Sun, Ning Zhou, Weipeng Wu, Weidong Zheng, Yukun WangMDA level of HBE cell lysates were measured with iron, 4-HNE, and MDA assay kit (#ab83366, #ab118970, ab238538; Abcam) respectively following the directions of the manufacturer. The levels of ROS and GSH were assessed

(18) Renal tubular peroxisomes are dispensable for normal kidney functionJCI InsightFebruary 22, 2022Camille Ansermet, Gabriel Centeno, Sylvain Pradervand, Dusan Harmacek, Andy Garcia, Jean Daraspe, Sai Kocherlakota, Myriam Baes, Yohan Bignon, Dmitri Firsovmagnification. ELISA and Trolox assay. 4-HNE was measured using Lipid Peroxidation (4-HNE) Assay Kit from Abcam (ab238538). Total and nonenzymatic antioxidant capacities were assessed by Trolox assay kit from Abcam (ab65329). Statistics. All

(19) Melatonin is a potential oncostatic agent to inhibit HepG2 cell proliferation through multiple pathwaysHeliyonJanuary 31, 2022Omar A. Ammar, Mohamed A. El-Missiry, Azza I. Othman, Maggie E. Amerroom temperature. After stopping the reaction with 100 μL of 2 M sulfuric acid stop solution (Abcam # ab238538, Cambridge, MA, USA), the absorbance of the samples at 450 nm was measured using a microplate reader.

(20) Growth Differentiation Factor 15 Regulates Oxidative Stress-Dependent Ferroptosis Post Spinal Cord Injury by Stabilizing the p62-Keap1-Nrf2 Signaling PathwayFrontiers in aging neuroscienceJanuary 1, 2022Xia M, Zhang Q, Zhang Y, Li R, Zhao T, Chen L, Liu Q, Zheng S, Li H, Qian Z, et al.the main products of lipid peroxidation, were measured by their detection kits (S0131M, Beyotime, and ab238538, Abcam) (Rong et al., 2021a). Briefly, for MDA detection, the samples were mixed with the working solution

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Lipid Peroxidation (4-HNE) Assay Kit from Abcam

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Lipid Peroxidation (4-HNE) Assay Kit from Abcam

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